Luciferase polyclonal antibody
产品名称： Luciferase polyclonal antibody
英文名称： Luciferase polyclonal antibody
产品编号： PAB11395
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Goat polyclonal antibody raised against native Luciferase.
- Immunogen:
- Native purified Photinus pyralis (firefly) Luciferase.
- Host:
- Goat
- Reactivity:
- Fruit fly
- Specificity:
- No reactivity is observed against Sea pansy (Renilla reniformis) luciferase.
- Form:
- Liquid
- Storage Buffer:
- In 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Native Purified Protein.
- Recommend Usage:
- ELISA (1:50000-1:200000)
Western Blot (1:5000-1:20000)
Immunohistochemistry (1:1000-1:5000)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
- Immunohistochemistry
- Luciferase immunostaining was analyzed in tibias from ovx ERE-luciferase mice taken 24 h after a 17-E2 injection. Positive luciferase staining was identified in hypertrophic chondrocytes (hc) (A), lining cells (lc) (B), osteoblasts (ob) (C), a subpopulation (10%) of osteocytes (ot) (upper arrow points at a positively stained osteocyte, whereas the lower depicts a negative osteocyte) (D), and megakaryocytes (E). Faint staining was found on the periosteal surface (p) (F). No background staining was seen when omitting the primary antibody (data not shown). The bar in the lower left corner represents 25 um. pc, Proliferative chondrocyte.
- Immunofluorescence
- NIH/3T3 cells transiently transfected with a Luciferase gene. Luciferase-positive cells were detected using Luciferase polyclonal antibody (Cat # PAB11395). Cells (25,000/well) were transiently transfected with a pLuc plasmid. After 2 days, the cells were fixed using 4% paraformaldehyde, permeabilized with 0.1% Triton® X-100, and blocked with 1% normal donkey serum. Cells were stained with 20 ug/ml Luciferase polyclonal antibody (Cat # PAB11395) in PBS for 2 hours followed by 1:200 dilution of donkey anti-goat IgG-FITC (green) for 1 hour. Cells were mounted using Vectashield with DAPI (blue) and visualized at 200X magnification with a fluorescent microscope.