BMPR1A polyclonal antibody
产品名称： BMPR1A polyclonal antibody
英文名称： BMPR1A polyclonal antibody
产品编号： PAB3832
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of BMPR1A.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human BMPR1A.
- Host:
- Rabbit
- Reactivity:
- Human, Mouse
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- BMP2 exposure results in decreased PTEN protein degradation and increased PTEN levels.
Waite KA, Eng C.Hum Mol Genet. 2003 Mar 15;12(6):679-84.
- 2.
- Germline mutations in BMPR1A/ALK3 cause a subset of cases of juvenile polyposis syndrome and of Cowden and Bannayan-Riley-Ruvalcaba syndromes.
Zhou XP, Woodford-Richens K, Lehtonen R, Kurose K, Aldred M, Hampel H, Launonen V, Virta S, Pilarski R, Salovaara R, Bodmer WF, Conrad BA, Dunlop M, Hodgson SV, Iwama T, Jarvinen H, Kellokumpu I, Kim JC, Leggett B, Markie D, Mecklin JP, Neale K, Phillips R, Piris J, Rozen P, Houlston RS, Aaltonen LA, Tomlinson IP, Eng C.Am J Hum Genet. 2001 Oct;69(4):704-11. Epub 2001 Aug 30.
- 3.
- Chromosomal localization of three human genes encoding bone morphogenetic protein receptors.
Astrom AK, Jin D, Imamura T, Roijer E, Rosenzweig B, Miyazono K, ten Dijke P, Stenman G.Mamm Genome. 1999 Mar;10(3):299-302.
- Applications
- Western Blot (Tissue lysate)
- Western blot analysis of BMPR1A polyclonal antibody (Cat # PAB3832) in mouse heart lysate. BMPR1A was detected as a specific doublet (arrows) at 60-65 kDa using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
- Western Blot (Cell lysate)
- Western blot analysis of BMPR1A polyclonal antibody (Cat # PAB3832) in CEM cell line lysates (35 ug/lane). BMPR1A (arrow) was detected using the purified polyclonal antibody.
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with BMPR1A polyclonal antibody (Cat # PAB3832) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human brainreacted with BMPR1A polyclonal antibody (Cat # PAB3832), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Immunohistochemistry
- Immunohistochemical staining of WT1, a podocyte-specific protein (top), and the BMPR1A receptor (middle) in podocytes of human kidney tissues gained from patients undergoing nephrectomy because of renal cancer. In contrast to the BMPR1A receptor, the BMPR2 receptor was not found in the glomerulus but can be clearly distinguished in monocytes infiltrating the glomerulus (bottom).
- Immunofluorescence
- Immunofluorescence analysis of BMPR1A polyclonal antibody (Cat # PAB3832) in frozen section of human brain tissue.
0.005 mg/mL primary antibody was followed by FITC-conjugated anti-rabbit antibody. FITC-conjugated anti-rabbit antibody emits green fluorescence. Blue counterstaining is Hoechst 33258.
This image kindly offered by Chunqing Zhang, Third Military Medical University.
- Application Image
- Western Blot (Tissue lysate)
- enlarge
- Western Blot (Cell lysate)
- enlarge
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- enlarge
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- enlarge
- Immunohistochemistry
- enlarge
- Immunofluorescence
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- ELISA
- Entrez GeneID:
- 657
- Protein Accession#:
- NP_004320
- Gene Name:
- BMPR1A
- Gene Alias:
- 10q23del,ACVRLK3,ALK3,CD292
- Gene Description:
- bone morphogenetic protein receptor, type IA
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The bone morphogenetic protein (BMP) receptors are a family of transmembrane serine/threonine kinases that include the type I receptors BMPR1A and BMPR1B and the type II receptor BMPR2. These receptors are also closely related to the activin receptors, ACVR1 and ACVR2. The ligands of these receptors are members of the TGF-beta superfamily. TGF-betas and activins transduce their signals through the formation of heteromeric complexes with 2 different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kD and type II receptors of about 70-80 kD. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding. [provided by RefSeq
- Other Designations:
- OTTHUMP00000020014,activin A receptor, type II-like kinase 3
- Related Disease
- Adenomatous Polyposis Coli
- Alzheimer Disease
- Anemia, Sickle Cell
- Anemia, sickle cell
- Bacteremia
- Chromosome Deletion
- Cleft Lip
- Cleft Palate
- Colon cancer
- Colonic Neoplasms
- Diabetes Mellitus, Type 2
- Gastrointestinal Neoplasms
- Genetic Predisposition to Disease
- Insulin Resistance
- Intestinal Polyposis
- Intestinal Polyps
- Kidney Failure, Chronic
- Liver Cirrhosis
- Neoplastic Syndromes, Hereditary