THY1 polyclonal antibody
产品名称： THY1 polyclonal antibody
英文名称： THY1 polyclonal antibody
产品编号： PAB3877
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of THY1.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human THY1.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Thy-1 expression in human fibroblast subsets defines myofibroblastic or lipofibroblastic phenotypes.
Koumas L, Smith TJ, Feldon S, Blumberg N, Phipps RP.Am J Pathol. 2003 Oct;163(4):1291-300.
- 2.
- THY1 expression is associated with tumor suppression of human ovarian cancer.
Abeysinghe HR, Cao Q, Xu J, Pollock S, Veyberman Y, Guckert NL, Keng P, Wang N.Cancer Genet Cytogenet. 2003 Jun;143(2):125-32.
- 3.
- The Thy-1/Thy-1 ligand interaction is involved in binding of melanoma cells to activated Thy-1- positive microvascular endothelial cells.
Saalbach A, Hildebrandt G, Haustein UF, Anderegg U.Microvasc Res. 2002 Jul;64(1):86-93.
- Applications
- Western Blot (Cell lysate)
- The THY1 polyclonal antibody (Cat # PAB3877) is used in Western blot to detect THY1 in HL-60 cell lysate.
- Western Blot (Cell lysate)
- Western blot analysis of THY1 polyclonal antibody (Cat # PAB3877) pre-incubated without (lane 1) and with (lane 2) blocking peptide in T-47D cell line lysate. THY1 (N-term) (arrow) was detected using the purified polyclonal antibody (1 : 60 dilution) .
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma reacted with THY1 polyclonal antibody (Cat # PAB3877) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Gene Pathway
- Related Disease