Product: DEDAF (Death Effector Domain Asso. Factor), Polyclonal
Cat. No: PC-106 (100 μg)
Background:
Apoptosis is related to many diseases and
induced by a family of cell death receptors and
their ligands, Cell death signals are transduced
by death domain (DD), death effector domain
(DED), and caspase recruitment domain (CARD)
containing molecules. Several molecules
including caspase and adaptor FADD contain
DEDs. A novel protein that interacts with DED of
caspase-8 and –10, and FADD was identified
recently and designated DEDAF for DED
associated factor. DEDAF is identical to the
transcriptional repressor RYBP. DEDAF/RYBP
is expressed in multiple tissues and cell lines.
DEDAF interacts with FADD and augments the
formation of CD95.FADD.caspase-8 complexes
at the cell membrane, and interacts with DEDcontaining
DNA binding protein (DEDD) in the
nucleus indicating it is involved in the regulation
of both cytoplasmic and nuclear events of
apoptosis.
Specificity:
Rabbit anti-DEDAF polyclonal antibody was
raised against a synthetic peptide
(TPKGDMSAVNDESF) corresponding to amino
acids 215 to 228 of human DEDAF. The
sequence is identical to that of mouse origin.
Positive control: Human A549 or HepG2 cell
lysate.
Species Reactivity:
Human, mouse and rat, others not tested.
Ig Isotype:
Rat IgG2a
Format:
100 μg in 200 μl of PBS containing 0.02%
sodium azide.
Storage and Stability:
Stable for 1 year when stored at 4°C.
Applications and Suggested Dilutions:

Western blot: Use at 0.5 to 1 μg/ml. A band of
approximately 32 kDa can be detected.
The optimal dilution for a specific application
should be determined by the researcher.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.