Product: Anti-14-16 kDa Nuclear Antigen, Clone IPO-38
Cat. No: MC-705 (100 μg)
Background:
The antibody recognizes a nuclear antigen that
is present in the cytoplasm and nuclei of
proliferating cells (paraformaldehyde fixation,
Triton X-100 permeabilization). Antigen is
absent from non-stimulated lymphocytes of
healthy human donors, but is present in early G1
stage cells after 12 hours of stimulation with
PHA. An increase of 400 is observed in cells
in mitosis (determined by analysis of K562 cells
synchronized with colcemide). IPO-38 does not
block binding of the Ki-67 antibody.
Specificity:
The antibody recognizes a 14-16 kDa nuclear
antigen (determined by Western blot analysis of
Raji cells).
Species Reactivity:
Human and mouse (determined by IIF), others
not tested.
Ig Isotype: IgM
Immunogen:
Hairy-cell leukemia cells
Hybridoma:
Mouse spleen cells fused with mouse myeloma
P3.X63Ag8.653A
Format:
1 mg/ml antibody in PBS, pH 7.2, purified on
protein A.
Storage and Stability:
+4°C short term, -20°C long term. Aliquot to
avoid freeze/thaw cycles
Applications and Suggested Dilutions:

ELISA

Indirect Immunofluorescence

Immunoprecipitation

Immunohistochemistry: Frozen sections
The optimal dilution for a specific application
should be determined by the researcher.
Reference:
1. Sidorenko, S.P., Shlapatskaya, L.N. Vetrova,
E.P., Yurchenko, O.V., Klenova, T.T.,
Mikhalap, S.V., Abramenko, I.V., and
Gluzman, D.F. (1994) Experimental Oncology
16:145-150.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.