Product: Anti-Fas Ligand mAb, clone 5G51
Cat. No.: MC-138 (100 μg)
Specificity:
This antibody is specific for the extracellular
domain of human Fas Ligand.
Cellular Localization:
Extracellular
Species Reactivity:
Human. Others not tested.
Ig Isotype:
Mouse IgG1
Immunogen:
Recombinant human Fas L (CD95L; APO-1L;
CD178) (extracellular domain)
Format:
100 μg at 1 mg/mL antibody in 0.15 M PBS, pH
7.2 with protein stabilizer. Epitope-affinity
purified
Purity:
³95% (SDS-PAGE).
Storage:
Store at 4°C. Do not freeze/thaw. Keep sterile.
Applications and Suggested Dilutions:

Functional Activity: Neutralizing. Recommend
a starting dilution of 1:100.

Flow cytometry

Immunohistochemistry: Frozen and paraffin
sections.
The optimal dilution for a specific application
should be determined by the researcher.
Figure 1: Flow cytometry detection of FasL expression in
stimulated H9 T cells.
Method: H9 T cells (1 x 106) were either left untreated (open
histogram) or stimulated with 10 ng/mL PMA and 1 μg/mL
ionomycin filled histogram). After 12 hours, cells were
harvested and stained with anti-FasL clone 5G51 (1 μg/mL),
followed by biotinylated goat anti-mouse IgG and
streptavidin-PE.
Figure 2: Inhibition of apoptosis in anti-CD3 stimulated
Jurkat T cells by anti-FasL.
Method: Jurkat JR cells (2 x 106) were stimulated with platebound
anti-CD3 antibodies (10 μg/mL) in the presence or
absence of the indicated concentrations of anti-FasL 5G51
or a control IgG1 mouse monoclonal antibody. After 48 hours
apoptosis was evaluated by flow cytometry analysis of PIstained
nuclei.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.