DiI-Ox- LDL DiI标记氧化型低密度脂蛋白
品牌：Solarbio | 货号：L8990




    
英文名称 : DiI-LLabeled oxidized low density lipoprotein, human
    
储存条件 : 2-8℃避光保存，请勿冷冻。
    
纯度 : 98%

 

 
 

    

        

            
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L8990-500ug
            
Solarbio
            
500ug
            
2500.00元
        
    

产品介绍
来源：人
浓度：1.0mg/ml 
 
DiI-LABELED OXIDIZED LOW DENSITY LIPOPROTEIN, HUMAN
Catalog No: L8990
Quantity: 500µg（micrograms） Protein/vial
Concentration: 3.2mg/ml (Protein)
Introduction ：
DiI-Ox-LDL, oxidized Low Density Lipoprotein, labeled with 1,1-dioctadecyl – 3,3,3,3-tetramethyl-indocarbocyanine perchlorate, labels both vascular endothelial cells and macrophages. It can be used to identify and/or isolate these cells from mixed cell populations and investigate uptake of modified LDL by different cell types. When cells are labeled with DiI-Ox-LDL, the lipoprotein is degraded by lysosomal enzymes and the DiI (fluorescent probe) accumulates in the intracellular membranes. Labeling cells with DiI-
Ox-LDL has no effect on cell viability. Pure cultures of vascular endothelial cells can be isolated from complex primary cultures using fluorescent activated cell sorting based on their increased metabolism of the DiI-Ox-LDL. Contaminating cell types (fibroblasts, smooth muscle, pericytes, epithelial cells) are not labeled. Macrophages can be differentiated from mixed cell populations (including endothelial cells) because they are more brightly labeled. Labeling endothelial cells with DiI-Ox-LDL has many advantages over labeling other endothelial cell associated antigens. The labeling procedure is one step, and once the cells are labeled, the fluorescent probe (DiI) is not removed by Trypsin. Both low density and confluent cultures of vascular endothelial cells are effectively labeled. No other cell type (other than macrophages) is labeled to the same level as vascular endothelial cells. Each lot of DiI-Ox-LDL is evaluated for the specific labeling of bovine
aortic endothelial cells and murine macrophages to assure consistent results. A complete labeling protocol is included with each shipment.
Storage & 
Stability:  This product is stable for 6 weeks after receipt when handled aseptically and stored at 2-8°C.
PROTECT FROM LIGHT AND NEVER FREEZE.
*Special Note:  After prolonged storage, some precipitate may be observed. This is normal for this product. Clarify out the aggregates by spinning in a microfuge for 2 minutes.
*Preparations of DiI-Ox-LDL are fairly unstable; plan your experiments in advance and use fresh material.
FOR RESEARCH USE ONLY
北京索莱宝科技有限公司 张净花 010-56371206  13426459985
 北京索莱宝供应各种低密度脂蛋白、高密度脂蛋白  欢迎咨询 
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因为此类产品保质期较短，所以都是现做的，订购后1-2个工作日可以发货