MAPK10 (N-term) Peptide
产品名称: MAPK10 (N-term) Peptide
英文名称: MAPK10 (N-term) Peptide
产品编号: P2390
产品价格: 0
产品产地: 台湾
品牌商标: Abnova
更新时间: null
使用范围: null
亚诺法生技股份有限公司(Abnova)
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- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of MAPK10.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human MAPK10.
- Host:
- Rabbit
- Reactivity:
- Human, Mouse
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Cyclin-dependent kinase 5 prevents neuronal apoptosis by negative regulation of c-Jun N-terminal kinase 3.
Li BS, Zhang L, Takahashi S, Ma W, Jaffe H, Kulkarni AB, Pant HC.EMBO J. 2002 Feb 1;21(3):324-33.
- 2.
- Head-to-head juxtaposition of Fas-associated phosphatase-1 (FAP-1) and c-Jun NH2-terminal kinase 3 (JNK3) genes: genomic structure and seven polymorphisms of the FAP-1 gene.
Yoshida S, Harada H, Nagai H, Fukino K, Teramoto A, Emi M.J Hum Genet. 2002;47(11):614-9.
- 3.
- Beta-arrestin 2: a receptor-regulated MAPK scaffold for the activation of JNK3.
McDonald PH, Chow CW, Miller WE, Laporte SA, Field ME, Lin FT, Davis RJ, Lefkowitz RJ.Science. 2000 Nov 24;290(5496):1574-7.
- Applications
- Western Blot (Tissue lysate)
- Western blot analysis of MAPK10 polyclonal antibody (Cat # PAB2784) in mouse spleen tissue lysate. MAPK10 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with MAPK10 polyclonal antibody (Cat # PAB2784) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
- Entrez GeneID:
- 5602
- Protein Accession#:
- P53779
- Gene Name:
- MAPK10
- Gene Alias:
- FLJ12099,FLJ33785,JNK3,JNK3A,MGC50974,PRKM10,p493F12,p54bSAPK
- Gene Description:
- mitogen-activated protein kinase 10
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This protein is a neuronal-specific form of c-Jun N-terminal kinases (JNKs). Through its phosphorylation and nuclear localization, this kinase plays regulatory roles in the signaling pathways during neuronal apoptosis. Beta-arrestin 2, a receptor-regulated MAP kinase scaffold protein, is found to interact with, and stimulate the phosphorylation of this kinase by MAP kinase kinase 4 (MKK4). Cyclin-dependent kianse 5 can phosphorylate, and inhibit the activity of this kinase, which may be important in preventing neuronal apoptosis. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq
- Other Designations:
- JNK3 alpha protein kinase,MAP kinase,OTTHUMP00000161180,OTTHUMP00000161182,OTTHUMP00000161183,c-Jun N-terminal kinase 3,c-Jun kinase 3,stress activated protein kinase JNK3,stress activated protein kinase beta
- Gene Pathway
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- Pancreatic cancer
- Pathways in cancer
- Toll-like receptor signaling pathway
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